Molecular analysis of a Phanerochaete chrysosporium lignin peroxidase gene.
Identifieur interne : 001009 ( Main/Exploration ); précédent : 001008; suivant : 001010Molecular analysis of a Phanerochaete chrysosporium lignin peroxidase gene.
Auteurs : I. Walther [Suisse] ; M. K Lin ; J. Reiser ; F. Suter ; B. Fritsche ; M. Saloheimo ; M. Leisola ; T. Teeri ; J K Knowles ; A. FiechterSource :
- Gene [ 0378-1119 ] ; 1988.
Descripteurs français
- KwdFr :
- ADN (analyse), ADN fongique (génétique), Cartographie de restriction (MeSH), Chrysosporium (enzymologie), Chrysosporium (génétique), Clones cellulaires (analyse), Deuteromycota (génétique), Données de séquences moléculaires (MeSH), Gènes (MeSH), Oxygénases (génétique), Séquence nucléotidique (MeSH).
- MESH :
- analyse : ADN, Clones cellulaires.
- enzymologie : Chrysosporium.
- génétique : ADN fongique, Chrysosporium, Deuteromycota, Oxygénases.
- Cartographie de restriction, Données de séquences moléculaires, Gènes, Séquence nucléotidique.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA.
- analysis : Clone Cells.
- enzymology : Chrysosporium.
- genetics : Chrysosporium, DNA, Fungal, Mitosporic Fungi, Oxygenases.
- Base Sequence, Genes, Molecular Sequence Data, Restriction Mapping.
Abstract
The basidiomycete fungus Phanerochaete chrysosporium produces a number of extracellular peroxidases which appear to be important for lignin degradation. We present here the isolation and complete nucleotide (nt) sequence of a gene (lpo) coding for lignin peroxidase (LPO), the coding region of which is identical to a lpo cDNA sequence which had previously been described [M. Tien and C.-P.D. Tu, Nature 326 (1987) 520-523]. The deduced amino acid (aa) sequence corresponds to 372 aa residues and the coding region is interrupted by eight short introns that range in size from 50 to 62 nt. Southern blot experiments using the cloned lpo gene as hybridization probe revealed a complex restriction fragment pattern, indicating that there are a number of lpo-related nucleotide sequences present in P. chrysosporium DNA which cross-hybridize. We also present data on the in vivo expression of the lpo genes and show that they are regulated at the RNA level and that the structure of the transcripts as judged from S1 experiments is complex. These data are consistent with the idea that there are a number of related lpo genes in P. chrysosporium which constitute a gene family.
DOI: 10.1016/0378-1119(88)90111-4
PubMed: 3240864
Affiliations:
Links toward previous steps (curation, corpus...)
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Suter</name></author><author><name sortKey="Fritsche, B" sort="Fritsche, B" uniqKey="Fritsche B" first="B" last="Fritsche">B. Fritsche</name></author><author><name sortKey="Saloheimo, M" sort="Saloheimo, M" uniqKey="Saloheimo M" first="M" last="Saloheimo">M. Saloheimo</name></author><author><name sortKey="Leisola, M" sort="Leisola, M" uniqKey="Leisola M" first="M" last="Leisola">M. Leisola</name></author><author><name sortKey="Teeri, T" sort="Teeri, T" uniqKey="Teeri T" first="T" last="Teeri">T. Teeri</name></author><author><name sortKey="Knowles, J K" sort="Knowles, J K" uniqKey="Knowles J" first="J K" last="Knowles">J K Knowles</name></author><author><name sortKey="Fiechter, A" sort="Fiechter, A" uniqKey="Fiechter A" first="A" last="Fiechter">A. Fiechter</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1988">1988</date><idno type="RBID">pubmed:3240864</idno><idno type="pmid">3240864</idno><idno type="doi">10.1016/0378-1119(88)90111-4</idno><idno type="wicri:Area/Main/Corpus">001004</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001004</idno><idno type="wicri:Area/Main/Curation">001004</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001004</idno><idno type="wicri:Area/Main/Exploration">001004</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular analysis of a Phanerochaete chrysosporium lignin peroxidase gene.</title><author><name sortKey="Walther, I" sort="Walther, I" uniqKey="Walther I" first="I" last="Walther">I. Walther</name><affiliation wicri:level="3"><nlm:affiliation>Institut für Biotechnologie, ETH-Hönggerberg, Zürich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institut für Biotechnologie, ETH-Hönggerberg, Zürich</wicri:regionArea><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName></affiliation></author><author><name sortKey="K Lin, M" sort="K Lin, M" uniqKey="K Lin M" first="M" last="K Lin">M. K Lin</name></author><author><name sortKey="Reiser, J" sort="Reiser, J" uniqKey="Reiser J" first="J" last="Reiser">J. Reiser</name></author><author><name sortKey="Suter, F" sort="Suter, F" uniqKey="Suter F" first="F" last="Suter">F. Suter</name></author><author><name sortKey="Fritsche, B" sort="Fritsche, B" uniqKey="Fritsche B" first="B" last="Fritsche">B. Fritsche</name></author><author><name sortKey="Saloheimo, M" sort="Saloheimo, M" uniqKey="Saloheimo M" first="M" last="Saloheimo">M. Saloheimo</name></author><author><name sortKey="Leisola, M" sort="Leisola, M" uniqKey="Leisola M" first="M" last="Leisola">M. Leisola</name></author><author><name sortKey="Teeri, T" sort="Teeri, T" uniqKey="Teeri T" first="T" last="Teeri">T. Teeri</name></author><author><name sortKey="Knowles, J K" sort="Knowles, J K" uniqKey="Knowles J" first="J K" last="Knowles">J K Knowles</name></author><author><name sortKey="Fiechter, A" sort="Fiechter, A" uniqKey="Fiechter A" first="A" last="Fiechter">A. Fiechter</name></author></analytic><series><title level="j">Gene</title><idno type="ISSN">0378-1119</idno><imprint><date when="1988" type="published">1988</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Chrysosporium (enzymology)</term><term>Chrysosporium (genetics)</term><term>Clone Cells (analysis)</term><term>DNA (analysis)</term><term>DNA, Fungal (genetics)</term><term>Genes (MeSH)</term><term>Mitosporic Fungi (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Oxygenases (genetics)</term><term>Restriction Mapping (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN (analyse)</term><term>ADN fongique (génétique)</term><term>Cartographie de restriction (MeSH)</term><term>Chrysosporium (enzymologie)</term><term>Chrysosporium (génétique)</term><term>Clones cellulaires (analyse)</term><term>Deuteromycota (génétique)</term><term>Données de séquences moléculaires (MeSH)</term><term>Gènes (MeSH)</term><term>Oxygénases (génétique)</term><term>Séquence nucléotidique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term><term>Clones cellulaires</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Clone Cells</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Chrysosporium</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Chrysosporium</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chrysosporium</term><term>DNA, Fungal</term><term>Mitosporic Fungi</term><term>Oxygenases</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN fongique</term><term>Chrysosporium</term><term>Deuteromycota</term><term>Oxygénases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Genes</term><term>Molecular Sequence Data</term><term>Restriction Mapping</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cartographie de restriction</term><term>Données de séquences moléculaires</term><term>Gènes</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The basidiomycete fungus Phanerochaete chrysosporium produces a number of extracellular peroxidases which appear to be important for lignin degradation. We present here the isolation and complete nucleotide (nt) sequence of a gene (lpo) coding for lignin peroxidase (LPO), the coding region of which is identical to a lpo cDNA sequence which had previously been described [M. Tien and C.-P.D. Tu, Nature 326 (1987) 520-523]. The deduced amino acid (aa) sequence corresponds to 372 aa residues and the coding region is interrupted by eight short introns that range in size from 50 to 62 nt. Southern blot experiments using the cloned lpo gene as hybridization probe revealed a complex restriction fragment pattern, indicating that there are a number of lpo-related nucleotide sequences present in P. chrysosporium DNA which cross-hybridize. We also present data on the in vivo expression of the lpo genes and show that they are regulated at the RNA level and that the structure of the transcripts as judged from S1 experiments is complex. These data are consistent with the idea that there are a number of related lpo genes in P. chrysosporium which constitute a gene family.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">3240864</PMID><DateCompleted><Year>1989</Year><Month>05</Month><Day>19</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0378-1119</ISSN><JournalIssue CitedMedium="Print"><Volume>70</Volume><Issue>1</Issue><PubDate><Year>1988</Year><Month>Oct</Month><Day>15</Day></PubDate></JournalIssue><Title>Gene</Title><ISOAbbreviation>Gene</ISOAbbreviation></Journal><ArticleTitle>Molecular analysis of a Phanerochaete chrysosporium lignin peroxidase gene.</ArticleTitle><Pagination><MedlinePgn>127-37</MedlinePgn></Pagination><Abstract><AbstractText>The basidiomycete fungus Phanerochaete chrysosporium produces a number of extracellular peroxidases which appear to be important for lignin degradation. We present here the isolation and complete nucleotide (nt) sequence of a gene (lpo) coding for lignin peroxidase (LPO), the coding region of which is identical to a lpo cDNA sequence which had previously been described [M. Tien and C.-P.D. Tu, Nature 326 (1987) 520-523]. The deduced amino acid (aa) sequence corresponds to 372 aa residues and the coding region is interrupted by eight short introns that range in size from 50 to 62 nt. Southern blot experiments using the cloned lpo gene as hybridization probe revealed a complex restriction fragment pattern, indicating that there are a number of lpo-related nucleotide sequences present in P. chrysosporium DNA which cross-hybridize. We also present data on the in vivo expression of the lpo genes and show that they are regulated at the RNA level and that the structure of the transcripts as judged from S1 experiments is complex. These data are consistent with the idea that there are a number of related lpo genes in P. chrysosporium which constitute a gene family.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Walther</LastName><ForeName>I</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Institut für Biotechnologie, ETH-Hönggerberg, Zürich, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kälin</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Reiser</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Suter</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Fritsche</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Saloheimo</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Leisola</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Teeri</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Knowles</LastName><ForeName>J K</ForeName><Initials>JK</Initials></Author><Author ValidYN="Y"><LastName>Fiechter</LastName><ForeName>A</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>M22720</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Gene</MedlineTA><NlmUniqueID>7706761</NlmUniqueID><ISSNLinking>0378-1119</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-49-2</RegistryNumber><NameOfSubstance UI="D004247">DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.13.-</RegistryNumber><NameOfSubstance UI="D010105">Oxygenases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.99.-</RegistryNumber><NameOfSubstance UI="C044391">ligninase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002912" MajorTopicYN="N">Chrysosporium</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002999" MajorTopicYN="N">Clone Cells</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005796" MajorTopicYN="N">Genes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003904" MajorTopicYN="N">Mitosporic Fungi</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010105" MajorTopicYN="N">Oxygenases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015183" MajorTopicYN="N">Restriction Mapping</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1988</Year><Month>10</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1988</Year><Month>10</Month><Day>15</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1988</Year><Month>10</Month><Day>15</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">3240864</ArticleId><ArticleId IdType="pii">0378-1119(88)90111-4</ArticleId><ArticleId IdType="doi">10.1016/0378-1119(88)90111-4</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suisse</li></country><region><li>Canton de Zurich</li></region><settlement><li>Zurich</li></settlement></list><tree><noCountry><name sortKey="Fiechter, A" sort="Fiechter, A" uniqKey="Fiechter A" first="A" last="Fiechter">A. Fiechter</name><name sortKey="Fritsche, B" sort="Fritsche, B" uniqKey="Fritsche B" first="B" last="Fritsche">B. Fritsche</name><name sortKey="K Lin, M" sort="K Lin, M" uniqKey="K Lin M" first="M" last="K Lin">M. K Lin</name><name sortKey="Knowles, J K" sort="Knowles, J K" uniqKey="Knowles J" first="J K" last="Knowles">J K Knowles</name><name sortKey="Leisola, M" sort="Leisola, M" uniqKey="Leisola M" first="M" last="Leisola">M. Leisola</name><name sortKey="Reiser, J" sort="Reiser, J" uniqKey="Reiser J" first="J" last="Reiser">J. Reiser</name><name sortKey="Saloheimo, M" sort="Saloheimo, M" uniqKey="Saloheimo M" first="M" last="Saloheimo">M. Saloheimo</name><name sortKey="Suter, F" sort="Suter, F" uniqKey="Suter F" first="F" last="Suter">F. Suter</name><name sortKey="Teeri, T" sort="Teeri, T" uniqKey="Teeri T" first="T" last="Teeri">T. Teeri</name></noCountry><country name="Suisse"><region name="Canton de Zurich"><name sortKey="Walther, I" sort="Walther, I" uniqKey="Walther I" first="I" last="Walther">I. Walther</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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